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expression vector ppiczαa  (Thermo Fisher)


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    Structured Review

    Thermo Fisher expression vector ppiczαa
    Expression Vector Ppiczαa, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ppicz%CE%B1a+expression+vector/pmc11724913-176-6-9
    Average 90 stars, based on 1 article reviews
    expression vector ppiczαa - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Activity Assay:

    Article Title: Hydrolysis Patterns of Xylem Tissues of Hardwood Pretreated With Acetic Acid and Hydrogen Peroxide
    Article Snippet: Xylanase (Xyl) of T. longibrachiatum (Cat. X2629) was purchased from Sigma- Aldrich. .. GH61-Auxiliary activity family 9 (AA9) copper-dependent lytic polysaccharide monooxygenases (LPMOs) of Gloeophyllum trabeum was cloned (GeneBank accession number: AEJ35168) and the recombinant enzymes were produced in Pichia pastoris with pPICZαA expression vector (Invitrogen, Carlsbad, CA, USA). .. The enzymewas purified with Ni-NTA column (Qiagen Hilden, Germany).

    Clone Assay:

    Article Title: Hydrolysis Patterns of Xylem Tissues of Hardwood Pretreated With Acetic Acid and Hydrogen Peroxide
    Article Snippet: Xylanase (Xyl) of T. longibrachiatum (Cat. X2629) was purchased from Sigma- Aldrich. .. GH61-Auxiliary activity family 9 (AA9) copper-dependent lytic polysaccharide monooxygenases (LPMOs) of Gloeophyllum trabeum was cloned (GeneBank accession number: AEJ35168) and the recombinant enzymes were produced in Pichia pastoris with pPICZαA expression vector (Invitrogen, Carlsbad, CA, USA). .. The enzymewas purified with Ni-NTA column (Qiagen Hilden, Germany).

    Article Title: Identification and Functional Characterization of a Novel Immunomodulatory Protein From Morchella conica SH
    Article Snippet: .. Synthesized FIP-mco CDS was digested and cloned into the corresponding restriction enzyme sites in pPICZαA expression vector (Invitrogen, California, USA), resulting in the recombinant plasmid pPICZαA-fipmco. ..

    Recombinant:

    Article Title: Hydrolysis Patterns of Xylem Tissues of Hardwood Pretreated With Acetic Acid and Hydrogen Peroxide
    Article Snippet: Xylanase (Xyl) of T. longibrachiatum (Cat. X2629) was purchased from Sigma- Aldrich. .. GH61-Auxiliary activity family 9 (AA9) copper-dependent lytic polysaccharide monooxygenases (LPMOs) of Gloeophyllum trabeum was cloned (GeneBank accession number: AEJ35168) and the recombinant enzymes were produced in Pichia pastoris with pPICZαA expression vector (Invitrogen, Carlsbad, CA, USA). .. The enzymewas purified with Ni-NTA column (Qiagen Hilden, Germany).

    Article Title: Identification and Functional Characterization of a Novel Immunomodulatory Protein From Morchella conica SH
    Article Snippet: .. Synthesized FIP-mco CDS was digested and cloned into the corresponding restriction enzyme sites in pPICZαA expression vector (Invitrogen, California, USA), resulting in the recombinant plasmid pPICZαA-fipmco. ..

    Article Title: The Fusarium graminearum cerato-platanins loosen cellulose substrates enhancing fungal cellulase activity as expansin-like proteins.
    Article Snippet: Accepted Manuscript The Fusarium graminearum cerato-platanins loosen cellulose substrates enhancing fungal cellulase activity as expansin-like proteins Alessandra Quarantin, Carla Castiglioni, Wilhelm Schäfer, Francesco Favaron, Luca Sella PII: S0981-9428(19)30107-X DOI: https://doi.org/10.1016/j.plaphy.2019.03.025 Reference: PLAPHY 5633 To appear in: Plant Physiology and Biochemistry Received Date: 14 November 2018 Revised Date: 27 February 2019 Accepted Date: 14 March 2019 Please cite this article as: A. Quarantin, C. Castiglioni, W. Schäfer, F. Favaron, L. Sella, The Fusarium graminearum cerato-platanins loosen cellulose substrates enhancing fungal cellulase activity as expansin-like proteins, Plant Physiology et Biochemistry (2019), doi: https://doi.org/10.1016/ j.plaphy.2019.03.025.. This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.

    Produced:

    Article Title: Hydrolysis Patterns of Xylem Tissues of Hardwood Pretreated With Acetic Acid and Hydrogen Peroxide
    Article Snippet: Xylanase (Xyl) of T. longibrachiatum (Cat. X2629) was purchased from Sigma- Aldrich. .. GH61-Auxiliary activity family 9 (AA9) copper-dependent lytic polysaccharide monooxygenases (LPMOs) of Gloeophyllum trabeum was cloned (GeneBank accession number: AEJ35168) and the recombinant enzymes were produced in Pichia pastoris with pPICZαA expression vector (Invitrogen, Carlsbad, CA, USA). .. The enzymewas purified with Ni-NTA column (Qiagen Hilden, Germany).

    Expressing:

    Article Title: Hydrolysis Patterns of Xylem Tissues of Hardwood Pretreated With Acetic Acid and Hydrogen Peroxide
    Article Snippet: Xylanase (Xyl) of T. longibrachiatum (Cat. X2629) was purchased from Sigma- Aldrich. .. GH61-Auxiliary activity family 9 (AA9) copper-dependent lytic polysaccharide monooxygenases (LPMOs) of Gloeophyllum trabeum was cloned (GeneBank accession number: AEJ35168) and the recombinant enzymes were produced in Pichia pastoris with pPICZαA expression vector (Invitrogen, Carlsbad, CA, USA). .. The enzymewas purified with Ni-NTA column (Qiagen Hilden, Germany).

    Article Title: Identification and Functional Characterization of a Novel Immunomodulatory Protein From Morchella conica SH
    Article Snippet: .. Synthesized FIP-mco CDS was digested and cloned into the corresponding restriction enzyme sites in pPICZαA expression vector (Invitrogen, California, USA), resulting in the recombinant plasmid pPICZαA-fipmco. ..

    Article Title: Natural gene variation in Cannabis sativa unveils a key region of cannabinoid synthase enzymes
    Article Snippet: .. Coding sequences of the WT THCAS (GenBank accession number AB057805 ) and associated mutants S355N, CONF and G376R were synthesized and subcloned into the pPICZαA expression vector (InvitrogenTM, Thermo Fisher Scientific) by GenScript® (Leiden, the Netherlands. ..

    Article Title: A Novel Anti-CD22 scFv.Bim Fusion Protein Effectively Induces Apoptosis in Malignant B cells and Promotes Cytotoxicity.
    Article Snippet: CD22 is a B-cell surface antigen which is highly expressed in cancerous B-cell lineages.. Anti-CD22 antibodies are currently under focus as promising biologics against hematologic B-cell malignancies.. Herein, we introduce a novel active recombinant anti-CD22 scFv.Bim fusion protein for targeting this cancerous antigen.

    Article Title: The Fusarium graminearum cerato-platanins loosen cellulose substrates enhancing fungal cellulase activity as expansin-like proteins.
    Article Snippet: Accepted Manuscript The Fusarium graminearum cerato-platanins loosen cellulose substrates enhancing fungal cellulase activity as expansin-like proteins Alessandra Quarantin, Carla Castiglioni, Wilhelm Schäfer, Francesco Favaron, Luca Sella PII: S0981-9428(19)30107-X DOI: https://doi.org/10.1016/j.plaphy.2019.03.025 Reference: PLAPHY 5633 To appear in: Plant Physiology and Biochemistry Received Date: 14 November 2018 Revised Date: 27 February 2019 Accepted Date: 14 March 2019 Please cite this article as: A. Quarantin, C. Castiglioni, W. Schäfer, F. Favaron, L. Sella, The Fusarium graminearum cerato-platanins loosen cellulose substrates enhancing fungal cellulase activity as expansin-like proteins, Plant Physiology et Biochemistry (2019), doi: https://doi.org/10.1016/ j.plaphy.2019.03.025.. This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.

    Article Title: The Xylanase Inhibitor TAXI-I Increases Plant Resistance to Botrytis cinerea by Inhibiting the BcXyn11a Xylanase Necrotizing Activity
    Article Snippet: .. The PCR amplicon, purified as above reported, was ligated into the EcoR I and Xba I sites of the pPICZαA expression vector (Thermo Fisher Scientific, Milan, Italy) and the ligation mixture was then used to transform E. coli competent cells. ..

    Article Title: Comparison of the Biochemical Properties and Roles in the Xyloglucan-Rich Biomass Degradation of a GH74 Xyloglucanase and Its CBM-Deleted Variant from Thielavia terrestris
    Article Snippet: The codons were optimized using GenSmartTM Codon Optimization tool (Version Beta 1.0, https://www.genscript.com.cn/gensmart-free-gene-codon-optimization.html ; accessed on 12 September 2019). .. The gene fragment was linked to the pPICZαA expression vector (Invitrogen, Carlsbad, CA, USA), located between the Eco RI and Not I sites. ..

    Article Title: A glycerol oxidase from Norway spruce ( Picea abies L. Karst.) expands biochemical and structural attributes of the GMC oxidoreductase superfamily
    Article Snippet: .. The Pa AOX1 was amplified from pJET1.2_ Pa AOX1 using the primers Pa AOX1pp_F and Pa AOX1pp_R and ligated to the pPICZαA expression vector (Thermo Fisher Scientific, Waltham, MA) between the KpnI and XbaI sites, transformed to DH5α, and sequenced. .. pPICZαA_ Pa AOX1 was linearized with MssI enzyme and transformed to Pichia pastoris wild type strain X-33 (Thermo Fisher Scientific, Waltham, MA) using electroporation.

    Synthesized:

    Article Title: Identification and Functional Characterization of a Novel Immunomodulatory Protein From Morchella conica SH
    Article Snippet: .. Synthesized FIP-mco CDS was digested and cloned into the corresponding restriction enzyme sites in pPICZαA expression vector (Invitrogen, California, USA), resulting in the recombinant plasmid pPICZαA-fipmco. ..

    Article Title: Natural gene variation in Cannabis sativa unveils a key region of cannabinoid synthase enzymes
    Article Snippet: .. Coding sequences of the WT THCAS (GenBank accession number AB057805 ) and associated mutants S355N, CONF and G376R were synthesized and subcloned into the pPICZαA expression vector (InvitrogenTM, Thermo Fisher Scientific) by GenScript® (Leiden, the Netherlands. ..

    Plasmid Preparation:

    Article Title: Identification and Functional Characterization of a Novel Immunomodulatory Protein From Morchella conica SH
    Article Snippet: .. Synthesized FIP-mco CDS was digested and cloned into the corresponding restriction enzyme sites in pPICZαA expression vector (Invitrogen, California, USA), resulting in the recombinant plasmid pPICZαA-fipmco. ..

    Article Title: Natural gene variation in Cannabis sativa unveils a key region of cannabinoid synthase enzymes
    Article Snippet: .. Coding sequences of the WT THCAS (GenBank accession number AB057805 ) and associated mutants S355N, CONF and G376R were synthesized and subcloned into the pPICZαA expression vector (InvitrogenTM, Thermo Fisher Scientific) by GenScript® (Leiden, the Netherlands. ..

    Article Title: The Fusarium graminearum cerato-platanins loosen cellulose substrates enhancing fungal cellulase activity as expansin-like proteins.
    Article Snippet: Accepted Manuscript The Fusarium graminearum cerato-platanins loosen cellulose substrates enhancing fungal cellulase activity as expansin-like proteins Alessandra Quarantin, Carla Castiglioni, Wilhelm Schäfer, Francesco Favaron, Luca Sella PII: S0981-9428(19)30107-X DOI: https://doi.org/10.1016/j.plaphy.2019.03.025 Reference: PLAPHY 5633 To appear in: Plant Physiology and Biochemistry Received Date: 14 November 2018 Revised Date: 27 February 2019 Accepted Date: 14 March 2019 Please cite this article as: A. Quarantin, C. Castiglioni, W. Schäfer, F. Favaron, L. Sella, The Fusarium graminearum cerato-platanins loosen cellulose substrates enhancing fungal cellulase activity as expansin-like proteins, Plant Physiology et Biochemistry (2019), doi: https://doi.org/10.1016/ j.plaphy.2019.03.025.. This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.

    Article Title: The Xylanase Inhibitor TAXI-I Increases Plant Resistance to Botrytis cinerea by Inhibiting the BcXyn11a Xylanase Necrotizing Activity
    Article Snippet: .. The PCR amplicon, purified as above reported, was ligated into the EcoR I and Xba I sites of the pPICZαA expression vector (Thermo Fisher Scientific, Milan, Italy) and the ligation mixture was then used to transform E. coli competent cells. ..

    Article Title: A glycerol oxidase from Norway spruce ( Picea abies L. Karst.) expands biochemical and structural attributes of the GMC oxidoreductase superfamily
    Article Snippet: .. The Pa AOX1 was amplified from pJET1.2_ Pa AOX1 using the primers Pa AOX1pp_F and Pa AOX1pp_R and ligated to the pPICZαA expression vector (Thermo Fisher Scientific, Waltham, MA) between the KpnI and XbaI sites, transformed to DH5α, and sequenced. .. pPICZαA_ Pa AOX1 was linearized with MssI enzyme and transformed to Pichia pastoris wild type strain X-33 (Thermo Fisher Scientific, Waltham, MA) using electroporation.

    Polymerase Chain Reaction:

    Article Title: The Fusarium graminearum cerato-platanins loosen cellulose substrates enhancing fungal cellulase activity as expansin-like proteins.
    Article Snippet: Accepted Manuscript The Fusarium graminearum cerato-platanins loosen cellulose substrates enhancing fungal cellulase activity as expansin-like proteins Alessandra Quarantin, Carla Castiglioni, Wilhelm Schäfer, Francesco Favaron, Luca Sella PII: S0981-9428(19)30107-X DOI: https://doi.org/10.1016/j.plaphy.2019.03.025 Reference: PLAPHY 5633 To appear in: Plant Physiology and Biochemistry Received Date: 14 November 2018 Revised Date: 27 February 2019 Accepted Date: 14 March 2019 Please cite this article as: A. Quarantin, C. Castiglioni, W. Schäfer, F. Favaron, L. Sella, The Fusarium graminearum cerato-platanins loosen cellulose substrates enhancing fungal cellulase activity as expansin-like proteins, Plant Physiology et Biochemistry (2019), doi: https://doi.org/10.1016/ j.plaphy.2019.03.025.. This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.

    Article Title: The Xylanase Inhibitor TAXI-I Increases Plant Resistance to Botrytis cinerea by Inhibiting the BcXyn11a Xylanase Necrotizing Activity
    Article Snippet: .. The PCR amplicon, purified as above reported, was ligated into the EcoR I and Xba I sites of the pPICZαA expression vector (Thermo Fisher Scientific, Milan, Italy) and the ligation mixture was then used to transform E. coli competent cells. ..

    Purification:

    Article Title: The Fusarium graminearum cerato-platanins loosen cellulose substrates enhancing fungal cellulase activity as expansin-like proteins.
    Article Snippet: Accepted Manuscript The Fusarium graminearum cerato-platanins loosen cellulose substrates enhancing fungal cellulase activity as expansin-like proteins Alessandra Quarantin, Carla Castiglioni, Wilhelm Schäfer, Francesco Favaron, Luca Sella PII: S0981-9428(19)30107-X DOI: https://doi.org/10.1016/j.plaphy.2019.03.025 Reference: PLAPHY 5633 To appear in: Plant Physiology and Biochemistry Received Date: 14 November 2018 Revised Date: 27 February 2019 Accepted Date: 14 March 2019 Please cite this article as: A. Quarantin, C. Castiglioni, W. Schäfer, F. Favaron, L. Sella, The Fusarium graminearum cerato-platanins loosen cellulose substrates enhancing fungal cellulase activity as expansin-like proteins, Plant Physiology et Biochemistry (2019), doi: https://doi.org/10.1016/ j.plaphy.2019.03.025.. This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.

    Article Title: The Xylanase Inhibitor TAXI-I Increases Plant Resistance to Botrytis cinerea by Inhibiting the BcXyn11a Xylanase Necrotizing Activity
    Article Snippet: .. The PCR amplicon, purified as above reported, was ligated into the EcoR I and Xba I sites of the pPICZαA expression vector (Thermo Fisher Scientific, Milan, Italy) and the ligation mixture was then used to transform E. coli competent cells. ..

    Amplification:

    Article Title: The Xylanase Inhibitor TAXI-I Increases Plant Resistance to Botrytis cinerea by Inhibiting the BcXyn11a Xylanase Necrotizing Activity
    Article Snippet: .. The PCR amplicon, purified as above reported, was ligated into the EcoR I and Xba I sites of the pPICZαA expression vector (Thermo Fisher Scientific, Milan, Italy) and the ligation mixture was then used to transform E. coli competent cells. ..

    Article Title: A glycerol oxidase from Norway spruce ( Picea abies L. Karst.) expands biochemical and structural attributes of the GMC oxidoreductase superfamily
    Article Snippet: .. The Pa AOX1 was amplified from pJET1.2_ Pa AOX1 using the primers Pa AOX1pp_F and Pa AOX1pp_R and ligated to the pPICZαA expression vector (Thermo Fisher Scientific, Waltham, MA) between the KpnI and XbaI sites, transformed to DH5α, and sequenced. .. pPICZαA_ Pa AOX1 was linearized with MssI enzyme and transformed to Pichia pastoris wild type strain X-33 (Thermo Fisher Scientific, Waltham, MA) using electroporation.

    Ligation:

    Article Title: The Xylanase Inhibitor TAXI-I Increases Plant Resistance to Botrytis cinerea by Inhibiting the BcXyn11a Xylanase Necrotizing Activity
    Article Snippet: .. The PCR amplicon, purified as above reported, was ligated into the EcoR I and Xba I sites of the pPICZαA expression vector (Thermo Fisher Scientific, Milan, Italy) and the ligation mixture was then used to transform E. coli competent cells. ..

    Transformation Assay:

    Article Title: A glycerol oxidase from Norway spruce ( Picea abies L. Karst.) expands biochemical and structural attributes of the GMC oxidoreductase superfamily
    Article Snippet: .. The Pa AOX1 was amplified from pJET1.2_ Pa AOX1 using the primers Pa AOX1pp_F and Pa AOX1pp_R and ligated to the pPICZαA expression vector (Thermo Fisher Scientific, Waltham, MA) between the KpnI and XbaI sites, transformed to DH5α, and sequenced. .. pPICZαA_ Pa AOX1 was linearized with MssI enzyme and transformed to Pichia pastoris wild type strain X-33 (Thermo Fisher Scientific, Waltham, MA) using electroporation.



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